Caryophyllene oxide exhibits anti-cancer effects in MG-63 human osteosarcoma cells via the inhibition of cell migration, generation of reactive oxygen species and induction of apoptosis

Zheng Pan, Shuan-Ke Wang, Xiao-Li Cheng, Xia-Wei Tian, Jing Wang

Abstract

The main objective of the present study was to evaluate the antitumor and apoptotic effects of caryophyllene oxide in MG-63 human osteosarcoma cells. Cell viability of these cells was evaluated by MTT assay while as in vitro wound healing assay was used to study the effect of caryophyllene oxide on cell migration. Fluorescence microscopy and transmission electron microscopy were used to study the changes in cell morphology once the cells undergo apoptosis. Caryophyllene oxide significantly led to cytotoxicity in MG-63 cells showing dose-dependent as well as time-dependent effects. Caryophyllene oxide led to an inhibition of wound closure significantly. At caryophyllene oxide doses of 20, 80 and 120 µM, the percentage of cell migration was shown to be 94.2, 67.1 and 14.8% respectively. With an increase in the caryophyllene oxide dose, the extent of apoptosis also increased characterized by cellular shrinkage, membrane blebbing, chromatin condensation and apoptotic body formation.

Video Clip of Methodology:

Cell viability assay: 2 min 22 sec   Full Screen   Alternative

 

Keywords

Apoptosis; Cancer; Caryophyllene oxide; Cell migration; MG-63 human osteosarcoma cell; Reactive oxygen species

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DOI: http://dx.doi.org/10.3329/bjp.v11i4.27517

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Copyright (c) 2016 Zheng Pan, Shuan-Ke wang, Xiao-Li Cheng, Xia-Wei Tian, Jing Wang

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Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is a single-blinded peer-reviewed, video component, open access official publication of the Bangladesh Pharmacological Society (BDPS). eISSN: 1991-0088. For contact: Editor-In-Chief