In vitro Propagation of the Biodiesel Plant Jatropha curcas L.

Authors

  • K. Kalimuthu Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore-641 028
  • S. Paulsamy 1Department of Botany, Kongunadu
  • R. Senthilkumar Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore-641 028
  • M. Sathya Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore-641 028

DOI:

https://doi.org/10.3329/ptcb.v17i2.3234

Keywords:

Jatropha curcas, Nodal explants, Cotyledons, Somatic Embryos, Multiple shoot formation

Abstract

The biodiesel plant, Jatropha curcas L. was micropropagated using nodal explants on MS supplemented with BAP (1.5 mg/l), Kn (0.5 mg/l) and IAA (0.1 mg/l). Somatic embryos were induced directly from green cotyledon explants on MS fortified with 2 mg/l of BAP. The results of histological studies showed that several layers of meristematic cells participated in the formation of somatic embryos. Rooting was effectively achieved on MS supplemented with IAA at 1.0 mg/l. The subsequent hardening experiment showed that the commercial medium containing a mixture of decomposed coir waste, perlite and organic compost in the ratio of 1:1:1 by volume was most effective, 80% plantlets survived.

 

Key words: Jatropha curcas, Nodal explants, Cotyledons, Somatic Embryos, Multiple shoot formation

 

D.O.I. 10.3329/ptcb.v17i2.3234

 

Plant Tissue Cult. & Biotech. 17(2): 137-147, 2007 (December)

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How to Cite

Kalimuthu, K., Paulsamy, S., Senthilkumar, R., & Sathya, M. (2009). In vitro Propagation of the Biodiesel Plant Jatropha curcas L. Plant Tissue Culture and Biotechnology, 17(2), 137–147. https://doi.org/10.3329/ptcb.v17i2.3234

Issue

Vol. 17 No. 2 (2007)

Section

Articles