Effect of Glutamine, Biotin and ADP on Micropropagation and Growth of Chrysanthemum hybridum, Gerbera jamesonii and Cordyline fruticosa In vitro

Authors

  • L Samarina Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi
  • T Kolomiets Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi
  • V Malyarovskaya Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi
  • S Gubaz Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi
  • N Platonova Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi

DOI:

https://doi.org/10.3329/ptcb.v26i1.29771

Keywords:

In vitro, Micropropagation, Growth, Microplants

Abstract

The effect of glutamine, biotin and adenosine diphosphate (ADP) on growth and cultivars micropropagation of three ornamental species was investigated. The addition of 10 ? 100 mg/l glutamine in culture media significantly increased rate of multiplication in Cordyline fruticosa and 100 mg/l glutamine showed the same effect for Gerbera jamesonii. Addition of glutamine did not show any effect on shoot length, root number and length in all the three species. Addition of 1 ? 3 mg/l biotin increased shoot length of Gerbera jamesonii but inhibited shoot length of Cordyline fruticosa and decreased root length of Chrysanthemum hybridum and Gerbera jamesonii. Addition of 5.0 mg/l ADP significantly increased multiplication rate of Cordyline fruticosa and 1.0 mg/l ADP showed similar effect for Gerbera jamesonii.

Plant Tissue Cult. & Biotech. 26(1): 97-104, 2016 (June)

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Author Biography

L Samarina, Laboratory of Plant Biotechnology, Physiology and Biochemistry, Russian Research Institute of Floriculture and Subtropical Crops, Jan Fabritsiusa str., 2/28, 354002 Sochi



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Published

2016-09-27

How to Cite

Samarina, L., Kolomiets, T., Malyarovskaya, V., Gubaz, S., & Platonova, N. (2016). Effect of Glutamine, Biotin and ADP on Micropropagation and Growth of Chrysanthemum hybridum, Gerbera jamesonii and Cordyline fruticosa In vitro. Plant Tissue Culture and Biotechnology, 26(1), 97–104. https://doi.org/10.3329/ptcb.v26i1.29771

Issue

Vol. 26 No. 1 (2016)

Section

Articles