<i>Agrobacterium</i>-Mediated Genetic Transformation of Potato for Abiotic Stress Tolerance

Authors

  • SN Begam Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • KM Nasiruddin Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • MS Haque Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • S Yasmin Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/pa.v19i2.16919

Keywords:

Potato, Agrobacterium mediated transformation, Abiotic stress

Abstract

Two potato varieties, Cardinal and Heera, were used to optimize the Agrobacterium-mediated genetic transformation system in potato for abiotic stress tolerance. MS medium supplemented with different concentrations and combinations of NAA and BAP were used to obtain callus induction from leaf explants of potato cv Cardinal and Heera. Maximum percentage of callus induction (95.83%) was observed in MS medium supplemented with 4 mg/L NAA and 2 mg/L BAP. The highest callusing was found in Cardinal and the lowest in Heera. Calluses were inoculated with Agrobacterium tumefaciens strain LBA4404 (pB1121) carrying CIPK sense gene, selectable marker gene nptII gene conferring resistance to kanamycin and the GUS reporter gene. Both the varieties were found GUS positive after inoculation and selection. Cardinal had maximum percentage of survived callus (25%) on selection media. Stable intregration and expression of the transgenes were confirmed by GUS histochemical assay.

DOI: http://dx.doi.org/10.3329/pa.v19i2.16919

Progress. Agric. 19(2): 37 - 43, 2008

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Published

2013-11-09

How to Cite

Begam, S., Nasiruddin, K., Haque, M., & Yasmin, S. (2013). <i>Agrobacterium</i>-Mediated Genetic Transformation of Potato for Abiotic Stress Tolerance. Progressive Agriculture, 19(2), 37–43. https://doi.org/10.3329/pa.v19i2.16919

Issue

Section

Crop Science