Molecular based prevalence of shigatoxigenic Escherichia coli in rectal swab of apparently healthy cattle in Mymensingh district, Bangladesh

Authors

  • Jayedul Hassan
  • K. H. M. Nazmul Hussain Nazir
  • Md. Shafiullah Parvej
  • Tazrin Kamal
  • Md. Tanvir Rahman

Keywords:

ehxA, non-O157, Rectal swab, STEC, Prevalence

Abstract

Objective: Cattle are regarded as the principle reservoir of O157 and non-O157 shigatoxigenic Escherichia coli (STEC). Spreading of the STEC to human is primarily happens through contaminated meat, milk and their byproducts. The present study was aimed to explore the occurrence of STEC in the rectal swab of apparently healthy cattle.

Materials and methods: A total of 60 E. coli isolates that were previously isolated from the rectal swab of cattle were used in this study. DNA were extracted from the isolates and screened by PCR to detect E. coli stx (stx1, stx2), ehxA and rfbO157 genes. Representative amplicons of the PCR products were sequenced. The prevalence of the STEC was determined based on the detection of STEC specific stx genes. The prevalence data were further analyzed by SPSS to elucidate any difference among different demographic groups of the study population.

Results: Overall, 43.33% (n=26/60) of the isolates were found carrying stx genes. Based on the presence of stx and ehxA genes, 6 different types of STEC were identified, of which 20% (n=12/26) were carrying both stx1 and stx2 genes. None of the isolates was positive for rfbO157. The PCR amplicons were sequenced, and the nucleotide sequences were deposited in GenBank (accession: KM596779-KM596784).

Conclusion: In this study, non-O157 STEC were found highly prevalent in the local cattle. This study suggests that the apparently healthy cattle may act as a potential source of STEC infection for humans.

http://doi.org/10.5455/javar.2017.d213                 

XML     PubReader

    

Downloads

Download data is not yet available.
Abstract
486
PDF
461

Author Biographies

Jayedul Hassan

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

K. H. M. Nazmul Hussain Nazir

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Shafiullah Parvej

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Tazrin Kamal

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Tanvir Rahman

Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Downloads

Published

2017-07-24

How to Cite

Hassan, J., Nazir, K. H. M. N. H., Parvej, M. S., Kamal, T., & Rahman, M. T. (2017). Molecular based prevalence of shigatoxigenic Escherichia coli in rectal swab of apparently healthy cattle in Mymensingh district, Bangladesh. Journal of Advanced Veterinary and Animal Research, 4(2), 194–199. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/33119

Issue

Vol. 4 No. 2 (2017)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).