Development of new spectrophotometric method for estimation of tenofovir disoproxil fumarate using MBTH reagent

Authors

  • Mannem Sri Varsha Department of Pharmaceutical Analysis & Quality Assurance G. Pulla Reddy College of Pharmacy Mehdipatnam, Hyderabad, Telangana 500028
  • N. Raghavendra Babu Department of Pharmaceutical Analysis & Quality Assurance G. Pulla Reddy College of Pharmacy Mehdipatnam, Hyderabad, Telangana 500028
  • Yenumula Padmavathi Department of Pharmaceutical Analysis & Quality Assurance G. Pulla Reddy College of Pharmacy Mehdipatnam, Hyderabad, Telangana 500028
  • P. Ravi Kumar Department of Pharmaceutical Analysis & Quality Assurance G. Pulla Reddy College of Pharmacy Mehdipatnam, Hyderabad, Telangana 500028

DOI:

https://doi.org/10.3329/icpj.v4i4.22620

Keywords:

3-methyl-2-benzothiazoline hydrazone, tenofovir disoproxil fumarate, spectrophotometric determination, ICH guidelines

Abstract

A new simple, sensitive and specific procedure has been developed for determination of tenofovir disoproxil fumarate in bulk and pharmaceutical dosage forms using MBTH reagent. The purpose of this analytical validation procedure is to validate it by laboratory experiments to prove that the method meets the minimum standards for laboratory use. 3-methyl-2-bezothiazoline hydrazone reacts with the secondary amine group of tenofovir in the presence of oxidizing agent, ferric chloride. The resulting apple green coloured chromogen when measured spectrophotometrically in visible region (i.e., 400-800nm) shows a maximum absorbance at 626.5nm. This method can be successfully applied for the determination of drug content in pharmaceutical formulations. The results of analysis have been validated statistically.

DOI: http://dx.doi.org/10.3329/icpj.v4i4.22620

International Current Pharmaceutical Journal, March 2015, 4(4): 378-381

 

Downloads

Download data is not yet available.
Abstract
950
PDF
1211

Downloads

Published

2015-03-18

How to Cite

Varsha, M. S., Babu, N. R., Padmavathi, Y., & Kumar, P. R. (2015). Development of new spectrophotometric method for estimation of tenofovir disoproxil fumarate using MBTH reagent. International Current Pharmaceutical Journal, 4(4), 378–381. https://doi.org/10.3329/icpj.v4i4.22620

Issue

Vol. 4 No. 4 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).