Volatile phytochemical composition of rhizome of ginger after extraction by headspace solid-phase microextraction, petrol ether extraction and steam distillation extraction

Zhan-nan Yang, Weiping Yang, Quancai Peng, Qiansong He, Yong Feng, Shiqiong Luo, Zhengwen Yu

Abstract

The sampling techniques headspace solid-phase microextraction (HS-SPME), petrol ether extraction (PEE) and steam distillation extraction (SDE) were compared for the GC-MS of volatile constituents present in ginger (Zingiber officinale). The effects of different parameters, such as extraction fibers, extraction time, extraction temperature and particle size ranges, on the HSSPME of rhizome of ginger were investigated. Zingiberene (53.12%) were predominant components of ginger samples obtained by HS-SPME whereas those levels were 39.01% in the same samples by PEE and 35.05% in those by SDE, respectively. HS-SPME with polydimethylsiloxane (PDMS) fiber was more selective and particularly efficient for the isolation of volatile phytochemical composition and afforded a higher yield of total compounds than PEE and SDE. The specifically compound isolated by SPME, which due to effective fiber, was much larger than that isolated by PEE or SDE. HS-SPME is a powerful tool for determining the volatile constitutes present in the traditional Chinese medicines.

Keywords: Ginger; Headspace solid-phase extraction; Petrol ether extraction; Volatile phytochemical; Zingiber officinale

Online 13 October 2009

DOI: 10.3329/bjp.v4i2.3232

Bangladesh J Pharmacol 2009; 4: 136-143 

Keywords

Ginger; Headspace solid-phase extraction; Petrol ether extraction; Volatile phytochemical; Zingiber officinale
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