Hepatoprotective effect of Cocculus hirsutus on bile duct ligation-induced liver fibrosis in Albino Wistar rats

Authors

  • Sagar P. Thakare Department of Pharmacology, Sigma Institute of Pharmacy, Bakrol, Waghodia, Baroda, Gujarat
  • Hitesh N. Jain Department of Pharmacology, Sigma Institute of Pharmacy, Bakrol, Waghodia, Baroda, Gujarat
  • Savita D. Patil Department of Pharmacology, R. C. Patel Institute of Pharmacy, Shirpur, Dhule, Maharashtra
  • Umesh M. Upadhyay Department of Pharmacology, Sigma Institute of Pharmacy, Bakrol, Waghodia, Baroda, Gujarat

DOI:

https://doi.org/10.3329/bjp.v4i2.2980

Keywords:

Bile duct ligation, Cocculus hirsutus, Glutathione, Hepatoprotective activity, Oxidative stress

Abstract

In this animal model (Wistar rats of either sex) common bile duct was ligated for 28 days. Rats were treated for 28 days with methanol extract of Cocculus hirsutus. On day 29, blood and liver were collected for biochemical estimation and histopathological studies. Bile duct ligation produced liver fibrosis with generation reactive oxygen species and induction of oxidative stress hence the different concentrations of methanolic extract of C. hirsutus were evaluated for in vivo glutathione reductase activity. On bile duct ligation the liver fibrosis was induced with significant rise in serum marker enzymes levels. The hydroxyproline accumulation caused by hydrophilic bile acids accompanied by elevated hepatic lipid peroxidation, and glutathione levels. Treatment with C. hirsutus decreased the elevated levels of serum marker enzymes showing hepatoprotection, which was further confirmed by histopathological results.

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Author Biography

Sagar P. Thakare, Department of Pharmacology, Sigma Institute of Pharmacy, Bakrol, Waghodia, Baroda, Gujarat

Lecturer

References

Balamurugan G, Muthusamy P. Observation of the hepatoprotective and antioxidant activities of Trianthema decandra Linn. (Vallai sharunnai) roots on carbon tetrachloride-treated rats. Bangladesh J Pharmacol. 2008; 3: 83-89.

Buetler E, Duron O, Kelly BM. Proved method for determination of blood glutathione. J Lab Clin Med. 1963; 61: 882-87.

DSOUZA M. Tribal Medicine. Social center, Ahmednagar, 1st ed. India, 1998, pp 143-44.

Ganapaty S, Dash GK, Suresh P. Diuretic, laxative and toxicity studies of Cocculus hirsutus aerial parts. Fitoterapia 2002; 73: 28-31.

Ganapaty S, Vijay K. Hypoglycemic activity of aerial parts of Cocculus hirsutus on alloxan-induced diabetes. Indian J Nat Prod. 2006; 22: 17-20.

Iqbal MJ, Dewan ZF, Choudhury SAR, Mamun MIR, Mashiuzzaman M, Begum M. Pre-treatment by hexane extract of Phyllanthus niruri can alleviate paracetamol-induced damage of the rat liver. Bangladesh J Pharmacol. 2007; 2: 43-48.

Jayakar B, Sangameswaran B. Anti-diabetic and spermatogenic activity of Cocculus hirsutus (L) diels. African J Biotech. 2007; 6: 1212-16.

Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. India, International Book Publishers, 1987, pp 86-87.

Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy, 26th ed. India, Nirali Prakashan, 2004, pp 101-10.

Lee TY, Chang HH, Chenjenn H. Herb medicine ameliorates hepatic fibrosis in bile duct ligation rats. J Ethnopharmacol. 2007; 109: 318-24.

Lin CC, Yen MH, Lo TS, Lin JM. Evaluation of the hepatoprotective and antioxidant activity of Boehmeria nivea var. nivea and B. nivea var. tenacissima. J Ethnopharmacol. 1998; 60: 9-17.

OECD Guidelines for the testing of chemicals revised draft guideline 425: Acute oral toxicity: 2001. Acute toxic class method.

Palsamy P, Malathi R. Evaluation of hypoglycemic and hypolipidemic activity of methanolic extract of Cocculus hirsutus (L) diels leves in streptozotocin-induced diabetes mellitus rats. Int J Biol Chem. 2007; 1: 205-12.

Panda BK, Mishra US. Antibacterial activity of the leaves of Cocculus hirsutus. Indian Drugs. 2007; 44: 108-10.

Rao VN, Patil N, Shalam MD. Hepatoprotective activity of alcoholic and aqueous extracts of leaves of Tylophora indica (Linn) in rats. Indian J Pharmacol. 2007; 39: 43-47.

Sanmugapriya E, Venkataraman S. Studies on hepatoprotective and antioxidant actions of Strychnos potatoram Linn. seeds on CCl4 induced acute hepatic injury in experimental rats. J Ethnopharmacol. 2006; 105: 154-60.

Saraswat B, Visen PKS, Patnaik GK, Dhawan BN. Ex vivo and in vivo investigations of picroliv from Picrorhiza kurrooa and in alcohol intoxication model in rats. J Ethnopharmacol. 1999; 66: 263-69.

Saxena AK, Singh B, Anand KK. Hepatoprotective effects of Eclipta alba on subcellular levels in rats. J Ethnopharmacol. 1993; 40: 155-16.

Tieppo J, Vercelino R, Dias AS, Marroni CA, Marroni N. Common bile duct ligation as a model of hepatopulmonary syndrome and oxidative stress. Arq Gastroenterol. 2005; 42: 244-48.

Torres-Durain PV, Miranda-Zamora R, Paredes Carbajal MC, Mascher D, Bie-Castillo J, Diaz-Zagoya JC. Studies on the preventive effect of Spirulina maxima on fatty liver development by carbon tetrachloride in the rat. J Ethnopharmacol. 1999; 64: 141-47.

Vogel GH, Vogel WH. Drug discovery and evaluation. Pharmacological Assays. 2nd ed. Germany. Springer. 2002, pp 936-44.

Wang G, Shen H, Rajaraman G, Roberts MS, Gong Y, Jiang P, Burczynsk F. Expression and antioxidant function of liver fatty acid binding protein in normal and bile-duct ligated rats. Eur J Pharmacol. 2007; 560: 61-68.

Wardi J, Reifen R, Aeed H., Zadel L, Avni Y, Bruck R. Beta carotene attenuates experimentally induced liver cirrhosis in rats. Isr Med Assoc J. 2001; 3: 151-54.

Yoshioka K, Mori A, Taniguchi K, Mutoh K. Cell proliferation activity of proliferating bile duct after bile duct ligation in rats. Vet Pathol. 2005; 42: 382-85.

Zafar R, Ali SM. Anti-hepatotoxic effects of root and root callus of Cichorium intybus. J Ethnopharmacol. 1998; 63: 227-31.

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Published

2009-09-06

How to Cite

Thakare, S. P., H. N. Jain, S. D. Patil, and U. M. Upadhyay. “Hepatoprotective Effect of Cocculus Hirsutus on Bile Duct Ligation-Induced Liver Fibrosis in Albino Wistar Rats”. Bangladesh Journal of Pharmacology, vol. 4, no. 2, Sept. 2009, pp. 126-30, doi:10.3329/bjp.v4i2.2980.

Issue

Vol. 4 No. 2 (2009)

Section

Research Articles

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