Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method

Authors

  • Vivek K. Bajpai Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749 http://orcid.org/0000-0003-0423-6195
  • Rajib Majumder Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749
  • Irfan A. Rather Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749 http://orcid.org/0000-0003-2752-0519
  • Kangmin Kim Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570752, Joellabuk-do (Jeonbuk)

DOI:

https://doi.org/10.3329/bjp.v11i3.27170

Keywords:

Exopolysaccharide, Lactic acid bacteria

Abstract

Lactic acid bacteria are classified Generally Recognized As Safe (GRAS) with most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits, such as modulation of immune response system and more importantly in food and pharma industries as a texturizer, viscosifer, emulsifier and syneresis-lowering agent. Its purification methodology involves: a) Extraction of cell-free supernatant from lactic acid bacteria; b) Denature of protein using trichloroacetic acid; c) Ethanol precipitation; d) Dialysis; and e) Freeze drying. However, depending on nature of research, compounds can be further purified using scanning electron microscopy (SEM), infrared spectrum (IR); and nuclear magnetic resonance (NMR) spectral analyses.

Video Clips of Methodology:

7 min 42 sec   Full Screen   Alternate

3 min 36 sec   Full Screen   Alternate

Downloads

Download data is not yet available.
Abstract
3540
Download
15259

Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Assistant Professor

Google Scholar Citation Index

Rajib Majumder, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Researcher

Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749

Assitant Professor

Google Scholar Citation Index

Kangmin Kim, Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570752, Joellabuk-do (Jeonbuk)

Professor

References

Cerning J, Renard CMGC, Thibault JF, Bouillanne C, Landon M, Desmazeaud M, Topisirovic L. Carbon source requirements for exopolysaccharide production by Lactobacillus casei CG11 and partial structure analysis of the polymer. Appl Environ Microbiol. 1994; 60: 3914-19.

Degeest B, Janssens B, De Vuyst L. Exopolysaccharide (EPS) biosynthesis by Lactobacillus sakei 0-1: Production kinetics, enzyme activities and EPS yields. J Appl Microbiol. 2001; 91: 470-47.

Grobben GJ, Sikkema J, Smith MR, De Bont JAM. Production of extracellular polysaccharides by Lactobacillus delbrueckii ssp. bulgaricus NCFB 2772 grown in a chemically defined medium. J Appl Bacteriol. 1995; 79: 103-07.

Gorska S, Jachymek W, Rybka J, Strus M, Heczko PB, Gamian A. Structural and immunochemical studies of neutral exopolysaccharide produced by Lactobacillus johnsonii 142. Carbohydr Res. 2010; 345: 108-14.

Ramchandran L, Shah NP. Effect of exopolysaccharides and inulin on the proteolytic, angiotensin-I-converting enzyme and ?-glucosidase-inhibitory activities as well as on textural and rheological properties of low-fat yogurt during refrigerated storage. Dairy Sci Technol. 2009; 89: 583-600.

Tallon R, Bressollier P, Urdaci MC. Isolation and characterization of two exopolysaccharides produced by Lactobacillus plantarum EP56. Res Microbiol 2003; 154: 705-12.

Wang CL, Huang TH, Liang TW, Fang CY, Wang SL. Production and characterization of exopolysaccharides and antioxidant from Paenibacillus sp. TKU023. N Biotechnol. 2011; 28: 559-65.

Downloads

Published

2016-06-02

How to Cite

Bajpai, V. K., R. Majumder, I. A. Rather, and K. Kim. “Extraction, Isolation and Purification of Exopolysaccharide from Lactic Acid Bacteria Using Ethanol Precipitation Method”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 573-6, doi:10.3329/bjp.v11i3.27170.

Issue

Vol. 11 No. 3 (2016)

Section

Visual Experiment

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).