Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5

Authors

  • Radhakrishnan Manikkam Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119 http://orcid.org/0000-0002-2689-6832
  • Shekar Ponnuswamy Department of Chemical Engineering, SSN College of Engineering, Chennai 603110
  • Jerrine Joseph Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119
  • Vanaja Kumar Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119

DOI:

https://doi.org/10.3329/bjp.v11i1.24238

Keywords:

Antitubercular, Forest soil, Streptomyces

Abstract

Extracellular pigment from the forest soil Streptomyces sp SFA5 was produced by submerged fermentation using yeast extract malt extract broth. Crude pigment from the medium was extracted using ethyl acetate. Antitubercular activity of the pigment was tested against M. tuberculosis H37Rv by microplate alamar blue assay and luciferase reporter phage assay. The pigment was also tested for inhibitory activity against M. tuberculosis lysine aminotransferase by colorimetric method. In both microplate alamar blue and luciferase reporter phage assay, the crude pigment showed activity against M. tuberculosis H37Rv at 125 and 250 µg/mL concentration, respectively. The M. tuberculosis lysine aminotransferase was inhibited at the IC50 value of 4.5 µg/mL concentration.

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Author Biography

Radhakrishnan Manikkam, Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119

Scientist

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Additional Files

Published

2015-12-25

How to Cite

Manikkam, R., S. Ponnuswamy, J. Joseph, and V. Kumar. “Antitubercular Activity of the Pigment from Forest Soil Streptomyces Sp SFA5”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Dec. 2015, pp. 138-40, doi:10.3329/bjp.v11i1.24238.

Issue

Vol. 11 No. 1 (2016)

Section

Research Articles

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