Inhibitory potential of Gossypium arboreum leaf extracts on diabetes key enzymes, α-amylase and α-glucosidase
DOI:
https://doi.org/10.3329/bjp.v8i2.14152Keywords:
α-Amylase, Diabetes, α-Glucosidase, Gossypium arboretum, TanninsAbstract
One of the antidiabetic therapeutic approaches is the reduction in gastrointestinal glucose production and absorption by inhibiting carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase. This present study evaluated the α-amylase and α-glucosidase inhibitory potential of the aqueous, ethanolic and acetone leaf extracts of Gossypium arboreum and to further determine the mode of inhibition of the enzymes using kinetic analysis. The aqueous and acetone extract exhibited potent α-amylase and α-glucosidase inhibitory activity with IC50 value of 10.10 mg/mL and 2.75 mg/mL respectively. These extracts are likely to contain non-competitive inhibitors of both enzymes with reduced Vmax values. The extracts were also found to contain phytochemicals such as tannins and steroids which may be responsible for the inhibitory effect. The results show that the aqueous and acetone extracts of G. arboreum leaf inhibited both α-amylase and α-glucosidase at relatively low concentrations and this could be the reason why the species is employed in the management of blood sugar related disorders by reasonable populations in Nigeria.
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Abo KA, Fred-Jaiyesimi AA, Jaiyesimi AEA. Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in South Western Nigeria. J Ethnopharmacol. 2008; 115: 67-71.
Ali H, Houghton PJ, Soumyanath A. Alpha-amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol. 2006; 107: 449-55.
Annan, K, Houghton PJ. Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana. J Ethnopharmacol. 2008; 119: 141-44.
Coutinho EM. Gossypol: A contraceptive for men. Contraception 2002; 65: 259-63.
Eloff JN. 1998. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol. 1998; 60: 1-8.
Essien EE, Aboaba SO, Ogunwande IA. Constituents and antimicrobial properties of the leaf essential oil of Gossypium barbadense (Linn.). J Med Plants Res. 2011; 5: 702-05.
Etuk EU, Bello SO, Isezuo SA. Mohammed BD. Ethnobotanical survey of medicinal plants used for the treatment of diabetes mellitus in north western region of Nigeria. Asian J Exp Biol. 2010; 1: 55-59.
Etuk EU, Mohammed BJ. Informant consensus selection method: A reliability assessment on medicinal plants used in north western Nigeria for treatment of diabetes mellitus. Afri J Pharmacol. 2009; 3: 496-500.
Gbolade AA. Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria J Ethnopharmacol. 2009; 121: 13539.
Hasrat JA, Peters L, Vlintinck AJ. Medicinal plants in Suriname: Hypotensive effect of Gossypium barbedense. J Pharm Pharmacol. 2004; 56: 381-87.
Kazeem MI, Akanji MA, Hafizur Rahman M, Choudhary MI. Antiglycation, antioxidant and toxicological potential of polyphenol extracts of alligator pepper, ginger and nutmeg from Nigeria. Asian Pac J Trop Biomed. 2012; 2: 727-32.
Kelly DE, Mandarino LJ. Fuel selection in human skeletal muscle in insulin resistance. Diabetes 2004; 40: 677-81.
Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effects of pine bark extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition 2005; 21: 756-61.
Kwon YI, Apostolidis E, Kim YC, Shetty K. Health benefits of traditional corn, beans and pumpkin? in vitro studies for hyperglycemia and hypertension management. J Med Food. 2007; 10: 266-75.
Kwon, Y.I., Apostolidis, E., Shetty, K. In vitro studies of egg plant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresource Technol. 2008; 99: 2981-88.
Lebovitz HE. Adjunct therapy for type 1 diabetes mellitus. Nat Rev Endocrinol. 2010; 6: 326-34.
Lee J M, Okumura MJ, Davis MM, Herman WH, Gurney JG. Prevalence and determinants of insulin resistance among U.S. adolescents: A population based study. Diabetes Care. 2006; 29: 2427-32.
Liu X, Kim JK, Li Y, Li J, Liu F, Chen X. Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-Li cells. J Nutr. 2005; 135: 165-71.
Mans DRA, Toelsie JR, Jagernath Z, Ramjiawan K, van Brussel A. Assessment of eight popularly-used plant derived preparations for their spasmolytic potential using the isolated guinea-pig ileum. Pharm Biol. 2004; 42: 422-29.
Mayur B, Sandesh S, Shruti S, Sung-Yum S. Antioxidant and ?-glucosidase inhibitory properties of Carpesium abrotanoides L. J. Med Plant Res. 2010; 4: 1547-53.
Mccue P, Shetty K. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asian Pac J Clin Nutr. 2004; 13: 101-06.
Min BR, Pinchak WE, Merkel R, Walker S, Tomita G, Anderson RC. Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens. Scientific Res Essay. 2008; 3: 66-73.
Oboh G, Akinyemi AJ, Ademiluyi AO. Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfaircaoccidentalis (fluted pumpkins) leaf. Asian Pac J Trop Biomed. 2012; 733-38.
Onal S, Timur S, Okutucu B, Zihnioglu F. Inhibition of α-glucosidase by aqueous extract of some potent antidiabetic medicinal herb. Prep Biochem Biotechnol. 2005; 35: 29-36.
Ono K. Hasegawa K, Naiki H, Yamada M. Anti-amylodogenic activity of tannic acid and its activity to destabilize Alzheimers beta-amyloid fibrils in vitro. Biochem Biophysacta. 2004; 1690: 193-202.
Saidu TB, Abdullahi M. Phytochemical determinations and antibacterial activities of the leaf extracts of Combretum molle and Gossypium arboretum. Bayero J Pure Appl Sci. 2011; 4: 13236.
Schwab S, Diem P. Oral hypoglycaemic agents in 2009. Ther Umsch. 2009; 66: 677-84.
Shai LJ, Masoko P, Mokgptho MP, Magano SR, Mogale AM, Boaduo N, Elof, JN. Yeast alpha glucosidase inhibitory and antioxidant activity of six medicinal plants collected in Phalaborwa, South Africa. South African J Bot. 2010; 76: 465-70.
Sofowara A. Medical plants and traditional medicine in Africa. Rep. Ibadan, Spectrum Books Ltd., 2006; pp 191-289.
Trease GE, Evans WC. Pharmacognosy. 4th ed. USA, W.B. Sounders, 1996, pp 243-83.
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