Antifungal activity of bioactive constituents and bark extracts of Rhododendron arboreum

Authors

  • Muhammad Nisar Institute of Chemical Sciences, University of Peshawar, Peshawar 25120
  • Sajid Ali Institute of Chemical Sciences, University of Peshawar, Peshawar 25120
  • Muhammad Qaisar Medicinal Botanic Center, PCSIR Laboratories Complex, Peshawar 25120
  • Syed Naeemuddin Gilani Medicinal Botanic Center, PCSIR Laboratories Complex, Peshawar 25120
  • Muhammad Raza Shah HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences,University of Karachi, Karachi 75270
  • Inamullah Khan Department of Pharmacy, University of Peshawar, Peshawar 25120
  • Gowhar Ali Department of Pharmacy, University of Peshawar, Peshawar 25120

DOI:

https://doi.org/10.3329/bjp.v8i2.14054

Keywords:

Antifungal activity, Rhododendron arboretum, Triperpenoid

Abstract

Rhododendron arboreum various fractions of bark as well as the isolated compounds were investigated against pathogenic fungi to provide evidence for the folkloric uses reported in the traditional system. Various solvent fractions and compounds were tested using agar well diffusion method at concentrations of 50, 25 and 12.5 mg/mL, the maximum inhibition recorded against fungi 17-32, 15-27 and 10-24 mm for methanol extract, 16-28, 14-26 and 10-22 mm to ethyl acetate extract, 17-21, 8-18 and 10-16 to chloroform extract and 8-15 and 8-12 mm to n-hexane extract respectively. Maximum inhibition at 10, 5 and 2.5 mg/mL of isolated triterpenoids was recorded against such as fungi 28-44, 25-42 and 20-40 mm for 3β-acetoxyurs-11,12-epoxy-13β,28-olide, 23-44, 20-42 and 15-40 mm for betulin and 17-40, 15-37 and 13-34 for lupeol respectively. It is concluded from the present study that the antifungal activity of the extracts may be due to the presence of 3β-acetoxyurs-11,12-epoxy-13β,28 - olide, betulin, lupeol and taraxerol.

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References

Arora C, Kaushik R. Fungicidal activity of plants extracts from Uttaranchal hills against soybean fungal pathogens. Alle J. 2003; 11: 217-28.

Brinkhaus B, Hentschel CR,Willich SN, Lehmacher WH, Eckhart GG. Herbal med, Keudell CV, Schindler G, Lindner M, Stützer H. Kohnen icine with curcuma and fumitory in the treatment of irritable bowel syndrome: A randomized, placebo-controlled, double-blind clinical trial. Scan J Gastroenterol. 2005; 40: 936-43.

Cakir NT, Guvenc A, Kokdil G, Duran NAG. Studies on the anatomical, phytochemical and antimicrobial properties of endemic Rhododendron smitnovii trautv. Ankara Universitesi Eczacilik Fakultesi Dergisi. 2005; 34: 165-82.

Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev. 2011; 24: 247-80.

Hamburger M, Hostettmann K. Bioactivity in plants: The link between phytochemistry and medicine. Phytochemistry 1991; 30: 3864-74.

Kiruba S, Mahesh M, Nisha SR, Miller PZ, Jeeva S. Phytochemical analysis of the flower extracts of Rhododendron arboreum Sm. ssp. nilagiricum (Zenker) Tagg. Asia Pac J Trop Biome. 2011; 1: S284-86.

Kurashige Y, Etoh JI, Handa T, Takayanagi K, Yukawa T. Sectional relationships in the genus Rhododendron (Ericaceae): Evidence from mat K and trn K intron sequences. Plant Syst Evol. 2001; 228: 1-14.

Mani TT, Badami S, Kumar A, Suresh B, Singh VK, Govil JN. Antioxidant and hepatoprotective activities of Rhododendron arboretum leaf. Phytopharmacol Therap Valu III. 2008; 3: 395-409.

Matin A, Khan MA, Ashraf MQ, Rizwana A. Traditional use of herbs, shrubs and trees of Shogran valley, Mansehra, Pakistan. Pakistan J Biol Sci. 2001; 4: 1101-07.

Nisar M, Ali S, Qaisar M. Preliminary phytochemical screening of flowers, leaves, bark, stem and roots of Rhododendron arboreum. Mid-East J Sci Res. 2011; 10: 472-76.

Quiroga EN, Sampietro AR, Vattuone MA. Screening antifungal activities of selected medicinal plants. J Ethnopharmacol. 2001. 74: 89-96.

Sahu PK, Rao CV, Verma N, Singh AP, Amresh G. Anti-inflammatory and anti nociceptive activity of Rhododendron arboreum. J Pharm Res. 2011; 3: 47-57.

Sparagano O, Foggett S. Diagnosis of clinically relevant fungi in medicine and veterinary sciences. Adv App Microbiol. 2009; 66: 29-52.

Verma N, Amresh G, Sahu PK, Mishra Neelam, Rao CV, Singh AP. Pharmacological evaluation of hyperin for antihyperglycemic activity and effect on lipid profile in diabetic rats. Indian J Exp Biol. 2013; 51: 65-72.

Verma N, Amresh G, Sahu PK, Mishra N. Protective effect of ethyl acetate fraction of Rhododendron arboreum flowers against carbon tetrachloride-induced hepatotoxicity in experimental models. Indian J Pharmacol. 2011a; 43: 291-98.

Verma N, Amresh G, Sahu PK, Mishra Neelam. Antidiarrheal potential of standardized extract of Rhododendron arboreum Smith flowers in experimental animals. Indian J Pharmacol. 2011b; 43: 689-98.

Additional Files

Published

2013-05-11

How to Cite

Nisar, M., S. Ali, M. Qaisar, S. N. Gilani, M. R. Shah, I. Khan, and G. Ali. “Antifungal Activity of Bioactive Constituents and Bark Extracts of Rhododendron Arboreum”. Bangladesh Journal of Pharmacology, vol. 8, no. 2, May 2013, pp. 218-22, doi:10.3329/bjp.v8i2.14054.

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Section

Research Articles