Hydroxysafflor yellow A suppresses oxidized low density lipoprotein induced proliferation of vascular smooth muscle cells

Authors

  • Lin Sheng Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Shaojie Bi Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Chao Cheng Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Jingbo Zhang Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033

DOI:

https://doi.org/10.3329/bjp.v7i2.10499

Keywords:

Hydroxysafflor yellow A, Oxidized low density lipoprotein, Mitogen activated protein kinase phospholipase 1, Smooth muscle cell

Abstract

To investigate the relationship between the suppression of Hydroxysafflor yellow A (HSYA) on the oxidized low density lipoprotein (ox-LDL) induced proliferation of vascular smooth muscle cells (VSMCs) and the mRNA and protein expression of extracellular signal-regulated protein kinase 1/2 (ERK1/2) and mitogen activated protein kinase phospholipase-1 (MAKP-1), VSMCs were treated with HSYA at 10 ?mol/L and/or ox-LDL at 35 mg/L for 48 h. MTT assay was done to measure cell survival rate, flow cytometry to detect cell cycle, reverse transcription PCR and Western blot to detect the expression of ERK1/2 and MAKP-1. When compared to cells treated with ox-LDL alone, the survival rate of cells treated with two reagents was reduced and the proportion of cells in G0/G1 phase significantly increased, with increased MKP-1 expression. The study suggests HSYA can inhibit VSMC proliferation via increasing MKP-1 expression, reducing p-ERK1/2 activity and suppressing cell cycle.

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References

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Published

2012-06-04

How to Cite

Sheng, L., S. Bi, C. Cheng, and J. Zhang. “Hydroxysafflor Yellow A Suppresses Oxidized Low Density Lipoprotein Induced Proliferation of Vascular Smooth Muscle Cells”. Bangladesh Journal of Pharmacology, vol. 7, no. 2, June 2012, pp. 87-93, doi:10.3329/bjp.v7i2.10499.

Issue

Section

Research Articles