Development of an Alternative Enzyme-assisted Dehairing Method of Animal Skins using Proteases from Bacillus licheniformis MZK05M9

Authors

  • Md Arafat Al Mamun Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000
  • Md Abir Hosain Department of Microbiology, University of Dhaka, Dhaka-1000
  • Sobur Ahmed Institute of Leather Engineering and Technology, University of Dhaka, Dhaka-1000
  • Fatema Tuj Zohra Institute of Leather Engineering and Technology, University of Dhaka, Dhaka-1000
  • Rajia Sultana Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000
  • Md Murad Khan Department of Microbiology, University of Dhaka, Dhaka-1000
  • Marufa Zerin Akhter Department of Microbiology, University of Dhaka, Dhaka-1000
  • Shakila Nargis Khan Department of Microbiology, University of Dhaka, Dhaka-1000
  • Md Mozammel Hoq Department of Microbiology, University of Dhaka, Dhaka-1000

DOI:

https://doi.org/10.3329/bjm.v32i0.28475

Keywords:

Bacillus licheniformis MZK05M9 (BlM9), eco-friendly, enzyme assisted process, dehairing

Abstract

The present study was aimed to develop an enzyme assisted dehairing method as an alternative and ecofriendly technique to reduce the use of harsh chemicals in leather manufacturing. For this purpose, two proteases namely alkaline protease (601 U/ml) and keratinase (132 U/ml) were produced by Bacillus licheniformis MZK05M9 (BlM9) in Soya molasses medium and Feather mill medium respectively, in 7.0 L bioreactor at pH 7.5 and 37°C. The cell free enzyme preparations were used together at 2.5% level in dehairing experiments. Three sets of experiments for dehairing of goat skins were performed with (1) enzymes; (2) enzymes and 5% lime (CaO) and (3) 2% sodium sulfide (Na2S) and 5% lime. The treatment with enzymes removed 85% of hair from goat skin after 24 hrs under mild shaking condition at room temperature where as the treatment with enzymes and 5% lime together resulted in 100% dehairing under similar conditions. Sodium sulfide along with lime also removed 100% hair faster (with 20 hrs) than other two treatments. The grain surface of the enzyme treated skin was smoother and silkier than that of the chemicals treatment as revealed by Scanning Electron Microscopy. Thus these results indicated that, the enzyme assisted method can be applied in largescale dehairing trial to reduce the use of harsh sodium sulfide.

Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 33-37

Downloads

Download data is not yet available.
Abstract
3008
PDF
1500

Author Biography

Md Arafat Al Mamun, Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000



Downloads

Published

2016-06-25

How to Cite

Mamun, M. A. A., Hosain, M. A., Ahmed, S., Zohra, F. T., Sultana, R., Khan, M. M., Akhter, M. Z., Khan, S. N., & Hoq, M. M. (2016). Development of an Alternative Enzyme-assisted Dehairing Method of Animal Skins using Proteases from Bacillus licheniformis MZK05M9. Bangladesh Journal of Microbiology, 32, 33–37. https://doi.org/10.3329/bjm.v32i0.28475

Issue

Section

Articles