Plant Extract Regulating Pectate Lyase Production in Soft Rotting Bacterial Isolates

Authors

  • Nazmun Nahar Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706
  • Md. Manjurul Haque Department of Environmental Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706
  • Md. Abu Ashraf Khan Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706
  • Md. Ashraful Haque Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706

DOI:

https://doi.org/10.3329/agric.v13i2.26594

Keywords:

Soft rotting bacterial isolates, pectate lyase, hyper-induction, plant extracts

Abstract

Soft-rotting bacteria caused diseases by secreting the plant cell-wall-degrading enzymes mainly pectate lyase (Pel). In this study, a total of 20 bacterial strains were isolated from soft rotted fruits and vegetables. Among them, 11 isolates produced soft rot symptom on potato tubers. All these isolates were partially characterized. When these isolates were tested for their Pel production in different media, the isolate P1 produced significantly higher amount of Pel than other isolates in M63 glycerol minimal medium. Interestingly, the isolate P1 grown on M63 glycerol minimal medium supplementing with 0.4% of polygalacturonic acid (PGA) and M63 glycerol minimal medium plus 0.4% of PGA and 1% of various plant extracts, Pel production was induced in M63 glycerol minimal medium containing 0.4% of PGA and hyper-induced in M63 glycerol minimal medium plus 0.4% of PGA and 1% of various plant extracts. However, Pel synthesis was not hyper-induced in high concentrations of potato extract. Thus, not only plant extracts but also concentrations of plant extract may be important for hyper-induction of Pel.

The Agriculturists 2015; 13(2) 81-88

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Published

2016-01-30

How to Cite

Nahar, N., Haque, M. M., Khan, M. A. A., & Haque, M. A. (2016). Plant Extract Regulating Pectate Lyase Production in Soft Rotting Bacterial Isolates. The Agriculturists, 13(2), 81–88. https://doi.org/10.3329/agric.v13i2.26594

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